Sentence examples for using selected cell lines from inspiring English sources

Here, we devised an in vitro model using selected cell lines, which recapitulates many features of EMT as observed in human breast cancer.

To check these results on the protein level Western Blot analysis was performed using selected cell lines.

Further, an in vitro infection assay using HPV pseudovirions was established to study the influence of inflammation on viral infectivity using selected cell lines.

It also underlines a necessity to determine cytotoxic effect by using rationally selected cell lines and by various methods targeting different cellular features, as the cell viability, proliferation, and membrane integrity, used in our case.

Total RNA from stably selected cell lines was extracted using TRIzol (Invitrogen) and treated with DNA-free DNase Treatment and Removal Reagents (Ambion, Austin, TX).

This, combined with the data from the literature, was used to select cell lines for which we could perform X chromosome gene copy number analysis.

To verify that MRP expression had not also been elevated in our daunorubicin selected cell line we used a Calcein-AM retention assay with the MRP modulator MK-571 [ 42] and compared MRP function to the adriamycin selected, MRP positive, HL-60ADR cell line [ 43].

We also performed similar experiments of knock down of these two genes using selected lymphoblastoid cell lines and it showed similar results as in the tumor cell lines (Figure 5A right panel, AraC p = 0.0074, gemcitabine p = 0.0085; Figure 5B right panel, AraC p = 0.001, gemcitabine p = 0.002).

We tested DNA from colon cancer cell lines to determine the reproducibility of the assay and to select cell lines to use as copy number controls.

Another important use of orthotopic models is to select cell lines that are more metastatic or more likely to metastasize to particular organs [165].

FASN inhibition-mediated effects on gene expression were measured using selected RT-PCR assays in additional tumor cell lines as well as in non-transformed cell types, including fibroblasts and endothelial cells.