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The kinetics of osmotic dehydration were investigated using 50%and65%5% sucrose solutions, using samples previously blanched by both stepwise and conventional means.
Very DIY, using samples.
They rarely use samples.
However, conventional SEM requires dehydration and metal coating of biological samples before observation, and because biofilms consist mainly of water, sample dehydration may influence the biofilm structure.
Caution was taken to avoid sample dehydration.
Following sample dehydration through graded alcohol concentrations (50%, 75 %, 96 % and 100, the aorta tissue was embedded into paraffin.
The sections of the formaldehyde fixed unembedded samples (cases 1 and 2) were processed for electron microscopy after osmification, using standard dehydration in ethanol and embedding in an Araldite Epon mixture.
In order to minimize the amount of deformation required in the brain alignment process, we prepared a standard brain (JFRC2013) using fixation and dehydration steps identical to those used to prepare our experimental samples.
Use sample test resources.
CONCLUSIONS: The true prevalence of dehydration among community-dwelling adults may be low or high, depending on the indicator(s) used to define dehydration.
Use sampling.
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