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Decalcified tissue sections were then prepared using routine procedures and stained with hematoxylin eosin (H&E) and Masson's trichrome (MT).
Paraffin embedded tissue in the form of tissue arrays were deparaffinized using routine procedures and samples were stained for C4BP as described [16].
All samples were processed using routine procedures and embedded in Paraplast (Sherwood Medical Ltd., Tullamore, Ireland).
Sciatic nerves fixed in 2%glutaraldehyde/1%% paraformaldehyde and post-fixed in 1% osmium tetroxide were Epon-embedded using routine procedures, and ultrathin sections were cut.
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The formalin-fixed samples were embedded in paraffin wax using routine procedures, sectioned at 4 μm and stained with hematoxylin and eosin.
The formalin fixed postmortem lung tissues were processed using routine procedures, initially stained with H&E, and then examined microscopically.
Histopathological assessment of livers were performed on sections (5 µm) cut from tissues embedded in paraffin and stained with hematoxylin and eosin (H&E) using routine procedures [65].
For morphometric analyses, 2- to 3-μm cross-sections of anterior compartment specimen were deparaffinized and stained with hematoxylin and eosin (H&E) using routine procedures as described previously (8).
One set of sections was stained with hematoxylin and eosin using routine procedures.
These sections were stained with hematoxylin and eosin using routine procedures.
These sections were stained with haematoxylin and eosin using routine procedures [ 50].
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