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Paraffin sections (4 μm thick) were processed using routine methods and stained with HE.
Haematoxylin- and eosin-stained slides were prepared from each tumour specimen using routine methods and then examined by light microscopy.
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Analysis of data used routine methods and data were analyzed using t-tests; a p value of 0.05 was considered statistically significant.
Pasteurellaceae were isolated and identified by using routine methods (25 ) and then stored at −80°C in 30% buffered glycerol in brain heart infusion agar (Hardy Diagnostics).
In brief, paraffin embedded tissue sections (7 μm) were cut and stained using routine methods, including hematoxylin and eosin, Gallyas silver and Luxol fast blue/cresyl violet.
Measurement of 24 h urinary sodium, potassium and creatine were undertaken using routine methods at baseline and follow-up as indices of adherence to the intervention and determined by assessors blinded to treatment allocation.
Sections were cut and stained by hematoxylin-eosin, von kossa, and elastin using routine methods [ 18].
These were prepared by placing a drop of active trichomonas culture onto a standard glass microscope slide; this was then air dried, alcohol-fixed and stained using routine methods.
Samples for histology were immediately fixed in 10% formalin and then processed and embedded in paraffin using routine methods.
Biochemical analyses were performed using routine methods in Clinical Laboratory Centre of the Kuopio University Hospital and in the Clinical Unit of the University of Kuopio.
The first half will be tested using routine methods in each site if clinically required and the other half will be frozen at −80°C for molecular diagnosis.
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