Sentence examples for using reverse transcriptase pcr from inspiring English sources

By using reverse transcriptase PCR (RT-PCR), 5′ and 3′ rapid amplification of cDNA ends (RACE) with designed, degenerate primers, a novel cDNA sequence was obtained.

We confirmed that all four genes are transcribed in adult N. vectensis using reverse transcriptase PCR (RT-PCR) (Fig. 1B).

Expression of SEC6 in the THE1-CIp10 control strain and tSEC6 after 2 and 4 h of growth in medium (with or without DOX) was assayed using reverse transcriptase PCR (RT-PCR) (see Fig. S1 in the supplemental material).

The detection of messenger RNA (mRNA) using reverse transcriptase PCR (RT-PCR) is becoming common practice for forensic body fluid identification.

All primers (Sigma, Australia) were optimized for use by amplification of cDNA using reverse transcriptase PCR and the resulting amplicons sequenced for confirmation.

We successfully isolated two genes in the terpene synthesis pathway from in vitro plantlets of A. malaccensis using reverse transcriptase PCR and specific primers.

In previous studies, TNF mRNA levels were showed to be similar between CL and ML patients whereas IL-10 mRNA was higher in ML than in CL lesions, when it was used reverse transcriptase PCR [ 28, 44].

To create real-time PCR standards, KDR and β-actin were amplified using reverse transcriptase-PCR using specific primers.

Viruses in the samples were detected using reverse transcriptase-PCR and confirmed by sequencing.

Viruses were detected using reverse transcriptase-PCR and confirmed by sequencing.

Using reverse transcriptase-PCR (RT-PCR) analysis, we investigated the expression of different GABA signaling molecules in the mouse taste system.