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Polymerase chain reaction (PCR) has been employed to amplify DNA fragments for molecular cloning, and is especially useful in cDNA cloning using reverse transcriptance PCR (RT PCR).
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Expression of the GLS gene was assessed by using reverse transcription-PCR (RT-PCR).
We examined the transcription of the OsRpl6-1 and OsRpl6-2 genes using reverse transcription-PCR (RT-PCR).
MAWD and MAWBP cDNAs were produced using reverse-transcription PCR (RT-PCR).
Results were confirmed using reverse transcription polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), and/or Sanger sequencing.
EXPERIMENTAL DESIGN: Galanin expression was examined using reverse transcription-polymerase chain reaction (PCR).
The detection of messenger RNA (mRNA) using reverse transcriptase PCR (RT-PCR) is becoming common practice for forensic body fluid identification.
The data obtained using macroarrays were compared with those obtained using reverse-transcription polymerase-chain reaction (RT-PCR).
RV-positive samples were serotyped using reverse transcriptase-PCR.
We also investigated PD-ECGF and VEGF gene expression using reverse transcriptase-PCR (RT-PCR).
Surfaces were evaluated for Nipah virus contamination by using reverse transcription PCR (RT-PCR).
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