The PTMC materials were highly biocompatible as determined by cell adhesion and proliferation studies using various relevant cell types (human umbilical vein endothelial cells (HUVECs), smooth muscle cells (SMCs) and mesenchymal stem cells (MSCs)).
The cytologist was blinded to the identity of the slides and differential cell counting was used to enumerate relevant cell types and thus quantify the percentage of micronucleated polychromatic erythrocytes (MN-PCEs) among total PCEs.
Cytological slides were examined blind, and differential cell counting was used to enumerate relevant cell types and thus quantify the frequency of micronucleated polychromatic erythrocytes (MN-PCEs) among total PCEs (>2,000 PCEs scored per slide).
Quite unexpectedly, generating mouse models using conditional approaches as well as GAA-based mouse models (Puccio et al., 2001; Miranda et al., 2002; Al-Mahdawi et al., 2006) was more successful than the design of stable cellular models using disease-relevant cell types.
Further studies will be required to examine whether the mice would present the same phenotype if other methods of conditional deletion of THOC5/FMIP in the relevant cell types were used.
Collectively, these results underscore the need for careful evaluation of ZnO nanoparticle effects across a spectrum of relevant cell types when considering their use for potential new nanotechnology-based biological applications.
False hits might be the result of using databases with data generated from multiple, often for a disease or trait phenotype not relevant cell types.
In order to verify the content of the network, we used publicly available data from experiments in which cell proliferation was modulated in the lung or lung relevant cell types.
Even more, using SSEA-4+ cell-subpopulations (SSEA-4+hASCs) residing within the adipose tissue, as a single-cellular source to obtain relevant cell types for bone regeneration, was also proposed.
Of note, the study was not conducted in clinically relevant cell types.
