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Plasma glucagon and C-peptide were measured by radioimmunoassay using reagents supplied by Linco Research (St . Louis MO).
Apolipoprotein AI (apoAI) and apolipoprotein B (apoB) were assayed using immunoturbidimetric methods, using reagents supplied by ABX Diagnostics.
N-terminal fragment BNP (NT-pro BNP) was measured using reagents supplied by Roche Diagnostics on an Elecsys 2010 analyzer (Roche Diagnostics, Castle Hill, Australia).
Apolipoprotein A1 concentrations were determined on an ILAB-650 (ILAB, Instrumentation Laboratories, Cheshire, UK) at King's College London using reagents supplied by Randox Laboratories Limited (County Antrim, UK).
Total activin A was measured by means of a highly specific solid-phase enzyme-linked immunometric assay using reagents supplied by DSL-France (Cergy-Pontoise, France).
When 100 ng of 448 gDNA samples were amplified using reagents supplied by MSI in kit form and the amplification carried out in-house, an average of 155 μg of MDA product was yielded in 150 μl reactions.
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Genomic DNA was eliminated by DNase treatment using reagents supplied with the kit.
Immunohistochemistry (IHC) staining for Jab1, EGFR, and S100A7 was performed using an automated tissue immunostainer (Ventana Medical Systems, Inc., Tucson, AZ, USA) and using bulk reagents supplied by the manufacturer (IHC protocol previously described [ 6, 21]).
Plasma triglycerides were analyzed on a cobas® 8000 analyzer Hoffman-La Rochee) using enzymatic reagents supplied by Hoffman-La Roche. Hoffman-La Roche
The concentration of βHB was determined on an automatic clinical analyser (Olympus AU400 Clinical Analyser, Tokyo, Japan) using the reagents supplied by Randox Laboratories (catalogue number RB 1007).
The concentration of urea was determined on an automatic clinical analyser (Olympus AU400 Clinical Analyser, Tokyo, Japan) using the reagents supplied by Olympus (catalogue number OSR6134).
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