Sentence examples for using reagents from the from inspiring English sources
RNA was reverse transcribed using reagents from the first-strand cDNA synthesis kit (Invitrogen).
After hybridization of the erm probe, the Southern blots were developed using reagents from the DIG DNA labeling and detection kit (Roche), according to the manufacturer's instruction.
DNA was extracted from the MBC's using a modified protocol previously described by Iovannisci, et al., 2006 [22], using reagents from the MasturePure DNA Purification Kit (Epincentre Technologies, Madison, WI, Cat. No. MCD85201).
RNAs were isolated from either transfected cells or papilloma tissue using the Trizol reagent (Invitrogen, Carlsbad, CA). 5 µg RNA from each sample (duplicates for each sample) was run out on a 1% formaldehyde gel using reagents from the Ambion Northern Max kit (Ambion, Austin, Texas).
Proteinase K digestion was performed using reagents from the MasterPure® Purification kit (Epicentre, Madison, WI).
Four cycles of PCR were performed using reagents from the Ovation Ultralow kit (Nugen).
Gentamicin was analyzed on an Architect Ci8200 analyzer (Abbott Laboratories, Abbott Park, IL, USA) using reagent from the same manufacturer (1P31).
CysC was analysed in serum using reagents from Dako and the Modular P 2551.
Monocyte nuclear extracts were prepared as previously described (15, 16), and nuclear factor (NF -κB p65 activity iNF -κBnuclear extracts were assessed using reagents from Bio-Rad using the Biop65x multiplex activityollowing the manuclearrextractstructions.
TAP and HS liquid media and agar plates were prepared in the lab using reagents from Fisher Scientific (Pittsburgh, PA) according to the protocol given in Gorman and Levine (1965) and Sueoka (1960), respectively.
The standard Illumina protocol was followed through using reagents from New England Biosciences with the following exceptions: samples were sheared by either nebulization or adaptive focused acoustics (Covaris), sequencing adaptors added after a-tailing contained an addition 4 bp nucleotide barcode on the 3' end allowing for the multiplexing of up to 4 samples per lane.
