Sentence examples for using random primers of from inspiring English sources

One microgram of total RNA from each sample was reverse transcribed using random primers of the High Capacity Reverse Transcription kit.

CP RNA was reverse transcribed using random primers of the Superscript First-strand Synthesis system for RT-PCR (Invitrogen).

Two micrograms of total RNA was reverse transcribed for each sample using random primers (final concentration of 5 μM) for metallothionein and gene-specific primers for the other three genes (0.5 μM final concentration, Table 3).

cDNA was generated using random primers with 2ug of total RNA using Superscript III reverse transcriptase according to the manufacturer's protocol (Invitrogen).

First-strand cDNA was synthesized by reverse transcription of 300 ng of total RNA in a final reaction volume of 20 μL using random primers and 200 units of SuperScript™ II Reverse Transcriptase (Invitrogen).

RNA samples were reverse-transcribed using random primers and specific regions of the viral cDNA were amplified by PCR and sequenced.

cDNA was generated using random primers and 20 U of reverse transcriptase (Promega).

Digested genomic DNA (2 μg) was labeled with amino-allyl-dUTP (Amersham-Pharmacia, Piscataway, NJ) using random primers in the presence of Klenow enzyme (Invitrogen Corp., Carlsbad, CA), followed by coupling to the Cy3 or Cy5 esters (Amersham-Pharmacia, Piscataway, NJ).

0.5 µg of total RNA isolated from injected larvae was reverse transcribed by 20 U of M-MuLV Reverse Transcriptase (Fermentas) using random primers in a final volume of 20 µl.

A total of 1.5 μg RNA was cDNA synthesized using random primers, and diluted to a total of 50 μl with nuclease free water to generate sufficient template for qPCR analysis.

We performed reverse transcription on 1 µg of aRNA using random primers from Roche and the SuperscriptII reverse transcriptase from Invitrogen.