Purified PCR products were labelled with [α-P] dCTP (250 μCi, 3000 Ci/mmol; Perkin Elmer) using random hexamers and free nucleotides were removed by gel filtration.
Using random sequences that mimic nucleotide variability along promoters, we obtain the p-value that corresponds to any particular Spf score.
Fluorescent nucleotides, Cyanine 3-dUTP (Cy3) or Cyanine 5-dUTP (Cy5) (GeneBeam, Enzo) respectively, were incorporated into first-strand cDNA by reverse transcriptase using random hexamer primers (Life Technologies) and Superscript III reverse transcriptase (Invitrogen).
Subsequently, we set all parameters of the nucleotide substitution model to the MLE values, and ran a heuristic tree search with an ML criterion, using random stepwise addition of samples and tree bisection reconnection.
Total RNA was extracted from brain homogenate, cDNA was synthesized by using random hexamer primers, reverse transcription PCR was conducted to amplify gene fragments, and nucleotide sequencing of genes was performed (4 ).
Several cryptographic applications use random numbers and pseudo random numbers.
Labeling of genomic DNA and hybridization to the Roche NimbleGen array were performed by the company (Roche NimbleGen) and used random priming to incorporate modified nucleotides by either amino-allyl or direct linkage to either of the two dyes used (Cy3 and Cy5).
The RNAs are reverse transcribed using labeled nucleotides and target specific, oligo dT, or random-sequence primers to create two labeled cDNA populations.
We have generated 10,000 random nucleotides using the same nucleotide composition of the real data and translated them into amino acids using the appropriate genetic code.
Further increasing the number of random nucleotides (5Ns and 6Ns) resulted in more bands (7 and 9 fragments, respectively).
