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cDNA synthesis was performed using random hexamers and SuperScript Reverse Transcriptase (Invitrogen).
RNA was reverse transcribed using random hexamers and the Multiscribe reverse transcriptase (Life Technologies).
mRNA was fragmented and reversed transcribed using random hexamers and SSII (Invitrogen) followed by second strand synthesis.
The first-strand cDNA synthesis was achieved by reverse transcription of RNA using random hexamers and SuperScript III Reverse Transcriptase (Invitrogen).
cDNA was synthesized using random hexamers and M-MLV (Invitrogen).
cDNA synthesis was performed using random hexamers, and Superscript III (Invitrogen) as per manufacturer's instructions.
RNAs were reversed transcribed into cDNA by using random hexamers and Superscript II Plus RNase H− Reverse Transcriptase (Invitrogen).
RNA was reverse transcribed using random hexamers and Moloney murine leukemia virus reverse transcriptase (Superscript II; Invitrogen).
Total RNA was prepared using Trizol Reagent (Invitrogen) and reverse transcribed using random hexamers and Superscript III reverse transcriptase.
First strand cDNA synthesis from 2 µg of total RNA was achieved using random hexamers and super-reverse transcriptase (Invitrogen).
From the amplified antisense RNA, double-stranded cDNA was synthesized using random hexamers and biotinylated oligo-dT.
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