Sentence examples for using random decamers and from inspiring English sources

RNA was reverse transcribed using random decamers and MMLVRT.

Reverse transcription of total RNA from each sample was carried out using random decamers and the RETROscript™ kit from Ambion.

A rapid amplification of cDNA ends (RACE) adapter was ligated to the product using T4 RNA ligase, and cDNA synthesis carried out using random decamers and M-MLV Reverse Transcriptase.

Three μg of total RNA from each tissue was used to perform three independent cDNA syntheses in a final volume of 10 μl, using random decamers and SuperScript II reverse transcriptase (Invitrogen).

The A260/A280 ratio and A260/A230 ratio of all samples were ≥ 2. cDNA was synthesized using random decamers (Ambion) and the SuperScript Double-Stranded cDNA Synthesis Kit (Invitrogen) according to the manufacturer's protocol.

cDNA of total RNA extracted from mice tissues, was synthesized by reverse transcription using random decamers, 0.5 µg total RNA, and M-MLV RT (Ambion, Denmark), according to manufacturer's instructions.

For the quantitative RT-PCR analysis total RNA (2 µg) was reverse transcribed using random decamers as primers (Ambion, cat.# 57226), and the MMLV-RT enzyme (Invitrogen, 28025-013).

RNA samples were treated with DNase I (DNA- free, Ambion, Austin, TX) and reverse-transcribed (2 μg) using random decamers (RETROscript, Ambion).

A 5′RACE RNA adapter was ligated to CIP/TAP treated RNA by T4 RNA ligase, and then reverse transcription was performed using random decamers.

Synthesis of cDNA was performed with the High-Capacity cDNA Archive Kit (Applied Biosystems) using random decamers.

RT PCR was performed as previously described (Moilanen et al, 2002) using random decamers (Ambion Europe Ltd., Cambridgeshire, UK).