Sentence examples for using quantitative pyrosequencing of from inspiring English sources

The methylation status of LINE-1-repetitive DNA element was analyzed using quantitative pyrosequencing of sodium bisulfite-converted DNA (31).

Using quantitative pyrosequencing, we detected a trend towards higher levels of the m.3365T>C mutation in RRF (72.2±24.4%, n=10) than non-RRF (56.5±28.8%, n=10, P=0.2049, two-tailed Student's t test).

Starting from an equal number of C. elegans and C. briggsae larvae, the proportion of both species was followed through time using quantitative pyrosequencing.

Therefore, the methylation level of LINE-1 sequences, highly repetitive DNA elements scattered throughout the human genome, was assessed using quantitative pyrosequencing.

For this purpose the global methylation level as well as gene-specific hypermethylation at 7 loci was assessed using quantitative pyrosequencing methodology.

The clinical relevance of PRAC methylation was determined in 136 human bladder specimens (eight normal controls [NCs] and 128 primary NMIBCs) using quantitative pyrosequencing analysis.

The mtDNA mutation load for specific alleles was assessed using quantitative pyrosequencing.

We first demonstrated using quantitative analysis (pyrosequencing) that CACNA2D3 expression varies with the methylation status of the CpG island.

Using quantitative PCR and pyrosequencing assay, the bacterial and methanotrophic communities in the top and bottom layers were analyzed along with the removal performance of landfill gases in the biocovers.

Expression levels were determined using quantitative reverse transcription polymerase chain reaction, and the percentage of DNA methylation was quantified by pyrosequencing.

Global DNA methylation was assessed by quantitative pyrosequencing of LINE-1 and Alu elements in two studies [ 118, 119].