These studies prompted a new line of investigation using quantitative methylation at repetitive elements in lymphocytes to evaluate the association of variation in environmental exposures with levels of what became accepted as a measure of global DNA methylation.
Using quantitative methylation specific PCR (qMSP), hypermethylation of the DCLK1 core promoter was observed in 134 out of 164 analyzed colorectal cancers and in none of 106 normal colorectal mucosa samples, resulting in a sensitivity and specificity of 82% and 100%, respectively (Fig. 1A).
DNA methylation analysis of SFRP2, WIF-1, DKK3 and SOX17 was performed using quantitative methylation specific PCR (qMSP) as described before [ 25, 30].
In this study, our aim is to explore whether methylation pattern at the promoter region of RAMP1 gene in peripheral leukocyte is associated with migraine using quantitative methylation approach.
The methylations of promoter were determined using quantitative methylation-specific PCR.
Levels of CLDN11 methylation and mRNA expression were measured in primary gastric cancer tissues, noncancerous gastric mucosae, and cell lines of gastric origin using quantitative methylation-specific PCR (qMSP) and quantitative reverse transcriptase-PCR (qRT-PCR), respectively.
The methylations of the promoter regions of DLEC1 and ACTB were determined using quantitative methylation-specific PCR.
We determined the promoter methylation of 6 genes and PIK3CA amplification using quantitative methylation-specific PCR (Q-MSP) and real-time quantitative PCR, respectively, and explore the association of promoter methylation with PIK3CA amplification in a large cohort of clinically well-characterized non-small cell lung cancer (NSCLC).
In this study, the hypermethylation of ADHFE1 was identified in CRC using quantitative methylation-specific polymerase chain reaction (QMSP).
Relative methylation of the NEFH CGI was analyzed in 132 RCC samples and 83 paired normal tissues using quantitative methylation-specific PCR.
