Sentence examples for using quantitative blotting of from inspiring English sources
Using quantitative blotting of Smc protein and replication origin DNA from cell extracts, we have estimated the average number of Smc protein to be around 30 dimers per replication origin in a fast growing population of cells.
Using quantitative Western blotting, we also examined time-dependent changes in the abundance of eight Wnt signaling proteins upon stimulation of HEK 293 cells with Wnt3a.
To estimate the specific activity of the CPG2 moiety of the fusion proteins, the concentrations of CPG2 protein in the conditioned media were measured using quantitative Western blotting.
To confirm the expression prolife of the molecules we observed in immuno- histochemistry studies, we measured the expression again by using quantitative western blotting or quantitative RT-PCR.
The expression levels of recombinant NNMT (NNMT-V5), EFNB2, synaptophysin, AktT protein, TH, tryptophan hydroxylase, ChAT, NeuN and AktPi were quantified using quantitative western blotting.
By using quantitative Western blot analysis, the effect of age on endogenous IGF2 levels was measured in the hippocampus of 15-month-old versus 7-month-old wild-type (WT) animals.
The intracellular concentrations of SepF and FtsZ were determined using quantitative western blot analyses as previously described (Ishikawa et al, 2006).
These predictions of the model were confirmed by experimental data using quantitative western blot and mass spectrometry analysis.
The steady-state protein levels of each Spy variant expressed at various IPTG concentrations were measured using quantitative western blots as described above.
To confirm these microarray results we examined the induction of a subset of CLEAR genes after ambroxol treatment using quantitative PCR, western blotting and enzymatic assays.
Therefore, we examined levels of GRP75/Mortalin and Calreticulin using quantitative fluorescent western blotting on human muscle biopsy samples obtained through EuroBioBank (see Methods).
