Sentence examples for using protocols provided by from inspiring English sources

The quantitative PCR assays were carried out using protocols provided by the manufacturers.

For double staining, CTA 157-2 was linked to NHS-Rhodamine (Pierce, Rockford, USA) using protocols provided by the manufacturers.

Enrichment of poly(A) RNA, synthesis of cDNA and construction of cDNA libraries were processed using protocols provided by Illumina, Inc. (San Diego, CA).

Sequenom MassARRAY quantitative methylation analysis was performed as previously described [ 32] using protocols provided by Sequenom (see Additional file 13 for a list of primers used).

For flow cytometric analysis of CTA 157-2 the antibody was linked to NHS-Dye 680 (MoBiTec, Göttingen, Germany) using protocols provided by the manufacturers.

Cell proliferation was determined by incubating the cells for 3 5 days in the presence of 0.1 mM bromo-2'-deoxyuridine (Oncogene Research, Cambridge, MA) followed by detection using protocols provided by the manufacturer.

Prior to stimulations, the cytotoxicity of all compounds was monitored, using propidium iodide (PI) (BD Biosciences, San Diego, CA) using protocol provided by the manufacturer.

Specific immunoreactive bands was identified and detected by enhanced chemiluminescence (ECL) using protocol provided by the manufacturer (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD).

Western blot analysis was performed as described previously20 using the protocols provided by the primary antibody suppliers.

Cell lines were cultured using standard protocols provided by ATCC.

Human cell lines were cultured using standard protocols provided by ATCC.