Sentence examples for using protocol described by from inspiring English sources
DNA fragmentation was evaluated by using protocol described by McGahon et al. (1995) with modification.
Total RNA for both NimbleGen microarray hybridization and real-time qPCR experiments was isolated using protocol described by Chang et al. [ 47].
Insect attack was done using protocol described by Babin et al. [ 55]. - P7, IMC47, UPA134 are Forastero genotypes originated from the Upper Amazonian region of Peru, known for their resistance to Phytophthora palmivora or P. megakarya.
The cDNA was synthesized using protocol described by a Thermo Scientific RevertAid Reverse Transcriptase kit (Thermo Fisher Scientific, Waltham, MA, USA). 1 μl of diluted cDNA (1 5) was used in each real time-PCR, using a THUNDERBIRD SYBR qPCR Mix (Toyobo, Life Science, Osaka, Japan), and an Illumina Eco Real-Time PCR Instrument (Illumina, San Diego, CA, USA).
Microarray hybridization was performed using the Affymetrix GeneChip Yeast Genome S98 Array using protocols described by Affymetrix (Santa Clara, CA).
Amplification and sequencing of nuclear 18S and 28S genes was carried out using protocols described by Struck et al. [ 24].
A set of physiological characteristics including catalase and oxidase tests and nitrate reduction were assessed using protocols described by Shieh et al. [ 17].
Three-week-old Balb/C female mice were anesthetized, and their inguinal mammary glands were cleared of host epithelium by using protocols described by Smith et al. [ 18- 20] (three mice per condition; six cleared mammary fat pads).
Excretory/secretory (ES) antigen of T. spiralis first stage larvae, used in ELISA and Western Blot (WB), was produced using the protocol described by Gómez-Morales et al. [ 13].
Complete ITS sequences of the isolated Microcystis strains were amplified using the protocol described by Janse et al. [54] using the primers CSIF and ULR.
Proteins were extracted using the protocol described by Adams et al. Western blotting was performed using the standard laboratory procedures with monoclonal antibody specific for 6His (Univ. Co, China).
