Sentence examples for using primers that target from inspiring English sources

In the second stage of amplification, the previously generated amplicons are amplified using primers that target the common sequence at the 5′-end of the primers [15] (Fig. 3a).

A highly variable region of the 16S rRNA gene is amplified using primers that target adjacent conserved regions, followed by direct sequencing of individual PCR products.

By using primers that target the conserved regions of the gene it is therefore possible to amplify DNA from most bacterial species.

Identification of the unique class 1 integron-IS26 gene arrangement in the bovine-derived strain D22 prompted the PCR interrogation of all strains with "non-amplifiable" gene cassette arrays in class 1 integrons using primers that target intI1 and IS26.

H. influenzae detection was done using primers that target the Outer membrane lipoprotein P2 (ompP2f, ompP2f:5'-GGTGCATTCGCAGCTTCAG-3' and ompP2r:5'GATTGCGTAATGCACCGTGTT-3'[ 25].

M. catarrhalis detection was done using primers that target the outer membrane protein copB gene, copBf:5'-GTGAGTGCCGCTTTTACAACC-3' and copBr:5'-TGTATCGCCTGCCAAGACAA-3' [ 23]. S. aureus was detected using primers that target the Thermonuclease (Tnase) encoding gene (nuc), nucf:5'-GCGandGATGGTGATACGGTT-3'5 anucrucr:5'-AGCCAAGCCTTGACGAACTAAAGC-3' [ 24].

C. leptum group DNA sequences were amplified using primers that targeted sequences in the 16S rRNA gene specific to that group.

Two other PCR assays were performed that were specific for sequences of O. tsutsugamushi and the genus Rickettsia by using primers that targeted a portion of the groEL gene because of its higher power of differentiation between closely related taxa.

We used primers that target fragments of the genes encoding for the large subunits of RubisCO form I (cbbL) and II (cbbM), as well as primers that target fragments of the small (aclB) and large (aclA) subunits of ACL.

To normalize the input DNA, we used primers that target the UBB promoter sequence that does not contain any CpG site.

The efficiency of DNA extraction and presence of inhibitors in samples that were negative by PCR were tested by using primers that targeted a fragment of the human β-globin gene as previously described (15 ).