Sentence examples for using primers that span from inspiring English sources

In order to test for E2-dependent binding of ERα to PCNA-ERE1 or PCNA-ERE2, we performed ChIP-PCR using primers that span each putative ERE.

The homozygous deletion of CDKN2A/B was confirmed by PCR analyses using primers that span each exon of the two genes.

AHA-4C1 (XIST+/Xist+) was derived from AHA-A5-2B (XIST+/Xist-) following treatment with 0.2 1.0 ug/ml 5-azacytidine and selection of Xist-expressing subclones as identified with RT-PCR using primers that span a splice junction (see supplementary Table S1 for primers).

Using primers that span exon junctions prevents the amplification of genomic DNA [ 63].

The DNA recovered from the immunoprecipitation was then amplified by PCR using primers that span the JunB-binding site.

RT-PCR was performed using primers that span an intron in MdACO to confirm that each RNA sample was free of genomic DNA contamination.

Lack of genomic contamination in the PCR amplification was confirmed using primers that spanned multiple exons, and the PCR products were sequenced.

To assess mRNA levels, cDNA was generated with a High-Capacity cDNA Archive kit (Applied Biosystems, Foster City CA) and amplified using primers that spanned exons 4 11, thereby amplifying both SYT/S and SYT/L transcripts.

The gene models of these effector candidates were further validated during the qRT-PCR analysis using primers that spanned the splice site junctions.

Assessment of mRNA expression by pigment cells was carried out as described previously (Eom et al., 2012) using primers that spanned the intron.

To make the CydX homologue-overexpression plasmids, the short genes were created through overlapping PCR using primers that spanned one half of each gene plus 15 nucleotides for a region of primer overlap.