Sentence examples for using primers that recognize from inspiring English sources
The specificity of the siRNA was confirmed using primers that recognize all PML isoforms.
As a control, PCR was also performed using primers that recognize the minimal promoter of mouse GAPDH.
The cells used in this study were confirmed to be of simian origin by karyotyping (10-87 LP, 10-87 HP, and 10-87 T) and by PCR using primers that recognize simian SINE sequences.
Breakpoints were cloned by long range PCR using primers that recognize sequences flanking the distal and proximal breakpoints, respectively, based on the customized array-CGH data.
Using primers that recognize the female-specific form, we observed that tra F mRNA increased ∼2.5-fold in the mutant and returned to near normal levels in the rescued mutant.
We next examined the transcriptional response of each EcR isoform to PoA and 20E using primers that recognize unique regions of each isoform and found that there was no difference in isoform regulation in Kc cells treated with either hormone.
Gene fusions were confirmed in stable transgenic lines by PCR using primers that recognized a sequence within the Citrine gene of the replacement construct combined with primers annealing with the genomic sequences flanking the 5′ region of the two identical PpHsp16.4a and PpHsp16.4b loci.
We used primers that recognize the flanking sequence of the barcoded cassettes as well as homology to the ho locus to produce PCR products for directed gene replacement into the ho locus in the MAT a WT strain BY4741.
The PCR signal from the M alleles was normalized to the signal from a control CYP26A1 region amplified by using primers that do not recognize any CpG (P774 sense: 5'- TTA GTG AAG GTT GTT TTG GGT-3' and 5'- AAT ACA AAT CCC AAA ACT TACT3').
However, amplification of netrin-1 mRNA using primers that selectively recognize the full-length (secreted) isoform of the protein showed that TNF-α does not significantly modify its gene induction, and in fact the ratio of secreted to total netrin-1 transcripts exhibited a trend towards reduction in response to TNF-α.
The ACE DD genotypes were confirmed by subjecting to a second independent PCR using the primers that recognize an insertion-specific sequence.
