Sentence examples for using primers that incorporated from inspiring English sources

The cDNA was created using primers that incorporated degeneracy surrounding the ATG, and was tagged with an N-terminal 6×-myc epitope to prevent binding of morpholino directed against the endogenous mpi mRNA (supplementary material Table S1).

The coding sequence for human CaM was amplified by PCR from the IMAGE clone (ID 2821489) [ 45] using primers that incorporated NcoI and XhoI restriction enzyme sites at the 5′ and 3′ ends respectively.

GFP sequence was PCR amplified from pcDNA3.1/NT-GFP using primers which incorporated 5' EcoRI and 3' XbaI sites.

To generate L22-GFP (C-terminal GFP fusion), L22 sequence was PCR amplified from pcDNA3-L22 using primers which incorporated 5' BamHI and 3' EcoRI sites.

L22 sequence was PCR amplified from pcDNA3-L22 using primers which incorporated 5' BamHI and 3' XbaI sites and cloned into BamHI and XbaI digested pCR3.1BAP to create pCR3.1BAP-L22 pCR3.1BAP-L22

The coding sequence for human UDP-glucose dehydrogenase was amplified by PCR from IMAGE clone [29] number 3916854 using primers which incorporated NcoI and XhoI restriction sites at the 5′ and 3′ ends of the sequence, respectively.

PCR was used to amplify the N-termini of mouse Cav1.4wt and Cav1.4nob2 from their appropriate cDNA templates, using primers CDRR69 (with incorporated BamHI restriction site; Table 1) and CDRR70 (with incorporated EcoRI site, Table 1).

The resulting cDNA underwent two rounds of PCR amplification (SsoFast EvaGreen supermix, BioRad) using GAPDH primers that incorporated the T7 sequence.

The genes for aprataxin residues 1 105 and APLF residues 1 106 were amplified using primer sets which incorporated 5′ BamHI and 3′ XhoI sites.

Three additional copies of the cMyc epitope were incorporated upstream of mCherry using primers KS3709 and KS3710.

For this, the target was PCR-amplified from pGEX-5X1/IL-24 pGEX-5X1/IL-24 pGEX-5X1/IL-24rs listedescribede 1, which haboveSbfI and a SgrAI site incorporated resusingg in primerslisted