Sentence examples for using primers that have from inspiring English sources

PCR was performed in a TGradient thermocycler (Biometra, Goettingen Germany), using primers that have been described previously [ 22].

An alternative approach is to perform directed PCR from cDNA using primers that have been designed based on ORF predictions, as has been successful for C. elegans [21].

A promoter-less wild-type EGFP was PCR amplified using primers that had a PciI site engineered in both the forward and reverse primers, a BclI site was engineered ahead of the PciI site in the reverse primer.

Gene deletions were made by first PCR amplifying a hygromycin B cassette (hphMX4) from pAG32 [27] using primers that had ends homologous to the flanking DNA of each gene to be deleted.

To illustrate the feasibility and effectiveness of this new method of primer design, we compared it with traditional methods using primers that had already been prelabeled with the fluorescent tag and those used in (1) multiplexed reactions or (2) with each primer pair run individually.

In the view of analytical technique, a standard PCR assay using primers that straddle the ITD mutation has been developed to detect length-altering mutations of ITD.

BRAF V600E mutations were detected using flanking primers that have been previously described [ 37].

Using common primers that have the ability to amplify both human and mouse K14, we observed a specific band of K14 with 202 bp size in tracheas (supplementary Figure 4s is available at Carcinogenesis Online).

The InDel and CAPS markers were derived from polymorphisms identified when sequencing PCR products from RH-1 and Herald using primers that we had designed on published M. truncatula BAC sequences at the tolerance locus.

The samples were amplified by PCR using the synthetic oligonucleotide primers that have been mentioned above.

For cloning NDRG1 full length gene in a mammalian expression vector NDRG1 was amplified from a prostate cDNA library (Clontech) using reverse primers that had a Flag sequence (DYKDDDDK) in-frame with the NDRG1 coding sequence,a stop codon was introduced after the FLAG sequence.