Sentence examples for using primers that encompass from inspiring English sources
The digested DNA was then used to amplify the promoter of interest using primers that encompass one or more Hpa II/Msp I sites.
To analyze RUNX3 binding to the promoter of miR-185, PCRs were carried out using primers that encompass RUNX3 BS1 or BS2 or BS3 of the miR-185 promoter.
To determine whether a decrease in the number of silent genes affects the amounts of the rRNA transcript, we measured 45S pre-rRNA synthesis by qRT-PCR using primers that encompassed the first rRNA processing site.
Gene specific primers that encompass one intron were defined for each transcript (except for HOXA9 gene) using the program Primer Express version 2. To design intron-spanning primers, putative intron-exon boundaries were deduced from the Genome Browser Database (http://genome.ucsc.edu/cgibin/hgGateway?hgsid=105081852&clade=vertebrate&org=Cow&db=0).
A P32 labelled probe was prepared by PCR, using primers encompassing the entire 5'nucleotidase coding sequence (1668 bp).
Genomic DNA was HpaII-digested, and resistance to digestion (i.e. CpG methylation) was measured by quantitative real-time PCR using primers encompassing HpaII sequences (CCGG) [1].
To simplify primer design and subsequent analysis, only alternative splicing events implicating cassette exons were verified using primers encompassing the alternative exons.
ChIP DNA was further analyzed by quantitative PCR using primers encompassing the regions of interest on the eIF4G1, CCND1, and CDK4 promoters.
It was verified that RNA obtained with this procedure was free of genomic DNA using primers 5afu4g11580 and 3afu4g11580 that encompasses three introns of the SOD2 gene.
The somatic status of the EGFR gene was investigated by PCR using primers specific for exons 18-21, encompassing the tyrosine kinase domain.
Genomic DNA was isolated from heterozygous y w 67c23; Top2 m/Top2 Df9043 larvae and PCR amplified using primers covering nine overlapping regions encompassing the Top2 protein-coding region.
