Sentence examples for using primers that covered from inspiring English sources

Alteration of selected color mutants in the six-gene cluster was screened by PCR using primers that covered the whole region.

In order to determine whether both loci of BRK1 are expressed in P. collenchymatum and P. eurystomum, RT-PCR and quantitative Real-Time PCR (qPCR) were performed using primers that cover polymorphisms in the exon (Additional file 3: Figure S1).

RT-PCR was performed on transcripts from Pold1+/− and Pold1exo/+ ES cells using two primers that covered the full-length Pold1 coding region.

DNA sequencing was performed using overlapping primers that covered the overall L-PCR fragments.

The cDNA was then used as a template to PCR-amplify the coding exons (exons 2 17) using overlapping primers that cover the exon-exon junctions (see Supplementary Table 1 (S1) in the Supplementary Material available online at http://dx.doi.org/10.1155/2014/370538).org/10.1155/2014/370538

To further confirm this finding, primers that covered the SALL4 promoter region were designed and used to map the SALL4 binding sites more precisely.

Fosmids with full-length RGHs, identified by amplifying with primers that covered the full-length genes, were then sequenced from both ends using the Big-Dye Terminator v3.1 cycle sequencing kit (Applied Biosystems) following the manufacturer's protocol.

We designed a set of primers that covered the entire human CA9 mRNA.

The first amplification was carried out using the Access RT-PCR System (Promega) with 6 different pairs of oligonucleotide primers that cover the entire HIV-1 genome.

Complete integration of this gene construct was tested using primer pairs that cover the region from the 35S-promoter of the ATDs element to flanking genes (either rolC [at right border of T-DNA] or nptII [at left border of T-DNA], see Figure 5b).

The design of a primer that covers all species within the domain Bacteria is impossible [ 33].