Sentence examples for using primers that bind from inspiring English sources

This idea was tested by 5' RACE using primers that bind downstream of the prrF1 terminator in the prrF1-prrF2 intergenic region (Figure 2A - PrrH.RACE1 and 2), yielding a product that, when sequenced, extended from the confirmed transcriptional start site of the PrrF1 RNA.

As cDNA was synthesised using oligo (dT) primers, both antisense and sense transcripts were polyadenylated and therefore detected in RT-PCR using primers that bind upstream and downstream of the third intron.

To identify these termini, JG068 DNA was directly sequenced using primers that bind the following four loci: within the left DTR, extending to the left terminus; immediately outside of the left DTR, extending to the left terminus; within the right DTR, extending to the right terminus; and immediately outside of the right DTR, extending to the right terminus.

To determine whether relapsing fever like spirochetes may infect I. ricinus ticks in our study sites, DNA of field-derived nymphs and adults was amplified by using primers that bind to a 16S rRNA gene fragment present in relapsing fever like spirochetes as well as Lyme disease spirochetes.

Given the clustering approaches described above, in combination with the observation that we used primers that bound to conserved regions within the V1R exon, we anticipated that closely related paralogs would be clustered together and thus we refrained from attempting to identify allelic variation within potentially non-homologous loci.

To compare the transfection efficiency and assess if similar amounts of the different expression plasmids entered BMC1 and MCF-7 cells, we performed real-time PCR using primers that specifically bind to the expression plasmids and the inserted genes (BORIS, Ets-1 and Sp1).

We used primers that bind in the flanking t-RNA genes to amplify the whole gene (1140 bp) in one fragment (data not shown).

(e) Colony PCR of transformants using primers that target cmx.

The transcription levels were determined for the pfkA gene using a set of primers that bind both to the cDNA of the endogenous pfkA gene as well as to the cDNA of the modified pfkA gene.

One documented source of allele-specific PCR amplification bias is the use of PCR primers that bind to sites containing a SNP [ 35].

Loop primers that bind to loop structures are used to shorten the reaction time of the LAMP assay [ 9].