Sentence examples for using primers that are from inspiring English sources

A second PCR was used to enhance the signal using primers that are extended at the 3' with one nucleotide (either A, T, C, or G) so a total of 16 primer combinations.

ACTB was used as a reference gene in the assay, using primers that are outside any CpG islands.

Our method could be applied to other cereal crops that have similar waxy mutant alleles using primers that are similar to those used in this study.

A total of 1490 Vidal clones that are present in the physical library were purportedly amplified using primers that are not compatible with current gene models.

The individual clones were completely sequenced using primers that are spanning the entire repeat length (approximately one primer every 300 bp, primer sequences available upon request).

As indicated in this study, long range PCR can be used effectively to isolate and sequence the LPS locus in diverse xanthomonads using primers that are targeted to the conserved metB and etfA genes.

PCR products were obtained using primers that were designed to amplify orthologous sequences from different tick species and genera.

The flaA and motAB genes were cloned from each of the three wild type strains' chromosomes using primers that were designed against the L. monocytogenes EGD-e genome sequence [19].

Bisulfite-modified genomic DNA was amplified using primers that were specific for methylated (M) and unmethylated (U) DNA.

To ensure that the gene had been amplified without introducing nucleotide errors, both strands were sequenced using primers that were specific for the T7 and SP6 promoters.

HLA-DRB1 genotyping was performed with initial group specific allele amplification using primers that were tagged with the M13 sequence (Additional files 1 and 2) [ 16– 16].