Sentence examples for using primers that amplified from inspiring English sources
The prevalence of R. felis-infection in individual C. felis lysates was assessed by qPCR using primers that amplified a portion of the 17-kDa antigen gene.
EGFR transcript expression was quantified by Real-Time RT-PCR, using primers that amplified exons 2 3 or exons 22 23 and thus allowed discrimination of wild-type EGFR transcripts and the EGFRvIII isoform.
The results, using primers that amplified the entire predicted ORF of the putative mouse psoriasin, are shown in Figure 2, with β-actin as a control in parallel.
Proteins lip13ss and pl2ss were produced using primers that amplified the protein minus signal peptides as the cloning of active lipases with their signal peptides was unsuccessful.
The BRI1 transcripts derived from the endogenous BRI1 and the transgene were shown to be inversely correlated with phenotypic severity by RT-PCR analyses using primers that amplified each gene specifically.
For the Malawian species, the genetically similar RH2aα and RH2aβ were analyzed together using primers that amplified both genes, so that qRT-PCR utilized six primer-probe sets (SWS1, SWS2b, SWS2a, RH2b, RH2a and LWS).
When amplification or sequencing failed, DNA was re-amplified and re-sequenced using primers that amplify smaller overlapping fragments and yield shorter sequences, but are still useful for specimen identification and sequence analysis [ 52, 53].
PCR amplification for genomic DNA and cDNA samples for IFIH1, (along with a minus-RT control) was carried out using primers that amplify exon 15 and thus are capable of amplifying both DNA and cDNA.
Left panel: Nested PCR analysis of ten ERH samples using primers that amplify HSV-1 glycoprotein D gene.
RNA analysis was carried out by RT-PCR using primers that amplify alternatively spliced forms of JCV early transcripts (Fig. 2A).
The final ChIP DNA was amplified by real-time PCR with SYBR green master mix RT-PCR reagent, using primers that amplify the ER binding region from the pS2 promoter.
