Sentence examples for using primers designed by from inspiring English sources

To screen for VHL mutations in thyroid aspirates, 2,5 μl of first-strand cDNA was used as a template for PCR using primers designed by us (F-5'-TCAGAGATGCAGGGACACAC-3', R-5'-TGACGATGTCCAGTCTCCTG-3').

The NASBA-ELISA system was developed by using primers designed by Loeffler J et al. (2001), who proved specificity of the primers and the use of bioth-probe also assured fine specificity for Aspergillus genus.

MLST was performed by PCR amplification and sequencing of seven housekeeping genes using primers designed by Enright et al [40].

Subsequently, bisulfite-treated DNA was amplified using primers designed by MethPrimer to span areas of CpG islands in the promoter or first intron [20].

Bisulfite-treated DNA was amplified using primers designed by MethPrimer to span areas of CpG islands in the promoter or first intron [15].

To confirm abnormal H3K9Ac of the promoters, we performed ChIP-qPCR using primers designed by Primer3 software from sequence information of the regions that were statistically significant at a P≤10−4 or less.

All amplification reactions were performed using primer designed by the aid of the Primer3 software.

Reverse transcription PCR was conducted on each pool by using primers designed to amplify all 4 DENV serotypes, followed by seminested PCR with serotype-specific primers (3 ).

All sequence was verified by classical dideoxy sequencing by using primers designed along the draft sequence.

PCR products were generated by using primers designed to include ends of the gene that was excised as well as flanking regions of the gene.

RT-PCR was performed by using primers designed in a previous study (14 ).