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Results were compared between cells patch-clamped using pipettes that contained either BAPTA solution or EGTA-BAPTA solution.
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In addition to spontaneous IPSCs, sSOCs could also be recorded using pipettes containing high Cl−.
To determine whether the cells in the white matter that respond to NAAG with a [Ca2+]i rise are oligodendrocytes, we patch-clamped cells whose morphology (assessed after Lucifer yellow filling from the patch pipette) corresponded to mature oligodendrocytes, using pipettes containing the ratiometric Ca2+-sensitive dye Fura-2.
Avoid using mouthwash that contains alcohol.
Do not use toothpaste that contains silica.
One should use toothpaste that contains fluoride.
Never use products that contain ammonia.
Loose patch recordings were done on Purkinje neurons using pipettes filled with external saline that were 6 7 MΩ in resistance.
Pressure polishing should also be useful in patch-clamp applications that benefit from using pipettes with blunt tips, such as perforated-patch whole-cell recordings, low-noise single channel recordings and experiments that require internal perfusion of the pipette.
Use a moisturizer that contains lactic acid.
Use a gel that contains hyaluronic acid.
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