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The qRT-PCR analysis of human brain samples was performed using our established methods [55].
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Peptide microarrays containing 132, 20-mer single-point variants of the YHNN peptide were generated using our established, in-house printing method [19].
Deep phenotyping included direct clinical assessments at multiple time points and review of lifetime medical records, with the use of our established methods (Fung et al. 2010; Vorstman et al. 2013; Swaby et al. 2011; Butcher et al. 2012, 2013; Cheung et al. 2014; Bassett et al. 2003).
With the use of our established method to purify larval NBs, and the development of new techniques for chromatin immunoprecipitation even from limited numbers of cells, it will become technically feasible to test the proposed network.
Embryoid bodies (EBs) were formed from the control and DMD iPSCs (Fig. 2A) to induce cardiomyocyte differentiation using our previously established method (Lin et al., 2012) (supplementary material Fig. S2a,b).
We used our previously established methods to quantify degradation of DQ-collagen IV on a per cell basis throughout the entire volume of SUM149 3D cultures [ 29].
Discrete choice methods will be used to establish maternal preferences, using established methods [ 31].
The validity of this simplified method was verified using other established methods and laboratory model tests.
ABPP-MudPIT analysis was performed using previously established methods.
(2) Metabolite signals in ITOCSY spectra were assigned using previously established methods.
Brains from 3 month old mice (n = 5) were homogenized using previously established methods (14).
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