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To eliminate the influence of the particle shape on the luminescence output color of Mn2+ ions, we have further synthesized rodlike hexagonal phase NaYF4 Ce,Mn NCs using our established method [21] in which the Ce3+-Mn2+ energy transfer also results in green Mn2+ luminescence at 500 nm (data not shown).
These procedures were carried out using our established method.
Using our established method, the femoral canal centerline could be fitted to a circle.
The cell death index was then determined using our established method.
The data used in the study were extracted from primary care computer systems and processed using our established method [ 34, 47].
Using our established method, CMs were obtained from HCM and control iPSCs with a high purity (∼90%), followed with whole transcriptome sequencing.
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We used our established method to calculate a Charlson co-morbidity index using diagnoses recorded in inpatient HES episodes, which started any time before the date of lung cancer diagnosis (Charlson et al, 1987; Rich et al, 2011b); this was analysed in four categories (0, 1, 2 3 and ⩾4).
Using our established criteria (see " Methods"), a total of 160 distinct N-glycosites within 83 glycoproteins were identified.
As this method of analysing cell cycle kinetics in the ovary is novel, we compared our findings with those obtained using the established method of flow cytometry.
Subsequent transfection to control and DMD hiPS cells was performed using an established method that allows well-controlled myogenic differentiation to occur at high efficiency26.
Limits of detection and quantification were calculated using an established method (MacDougall & Crummett 1980).
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