As a positive marker, we also used nuclear protein of mice treated with 3MC.
Using nuclear protein extracts from lung tissue, phosphorylation of STAT-6 could be detected following injection of IL-4 into adult wild-type mice (R.O).
We then tested oligonucleotides with each allele of each SNP in gelshift assays using nuclear protein extracts from rat hypothalamus.
The nuclear protein in TNC samples were extracted using Nuclear and Cytoplasm Protein Extraction kit (Catal. No. WLA020, Wanleibio, China).
Instead of using nuclear extracts, we used proteins translated in vitro from the same mRNA used for the microarray experiments.
We also confirmed that nuclear expression of RUNX3 by using nuclear and cytoplasmic fraction of HNSCC cell lines, indicating no protein mislocalization (data not shown).
The cytoplasmic and nuclear proteins were extracted using nuclear extraction kit (Imgenex Corporation, San Diego, CA, USA).
Studying dynamic aspects of protein structure using nuclear magnetic resonance spectroscopy, for example, is still sufficiently costly and time consuming as to prevent its use in the same scale as X-ray crystallography.
Dr. Kurt Wüthrich, 64, a professor of biophysics at the Swiss Federal Institute of Technology in Zurich, received the other half of the prize for using nuclear magnetic resonance -- the same underlying science as in the familiar medical M.R.I. -- to map the structure of proteins.
In cancer tissue that undergoes the LOH event that reduces a gene dose, the remaining gene copies have the increased possibility of using the nuclear proteins and this would lead to the demethylation of transitional-CpG segments [ 3, 4, 12].
