Sentence examples for using nuclear extracts of from inspiring English sources

EMSA analysis using nuclear extracts of E2-treated cells and natural sequences, including these putative EREs, indicated that ERβ – the only isoform expressed in U-937 cells – specifically recognized both EREs because ERβ DNA complexes were efficiently competed by the corresponding unlabelled probe and supershifted by the anti-human ERβ (L-20) antibody.

To determine whether GATA4/6 were recruited to the UP1B and UP2 promoters, we performed EMSA using nuclear extracts of RA-treated ESCs and spontaneously differentiating controls.

EMSA using nuclear extracts of mouse gastric tissues document that Hp-infection significactivatedivated NFκB (Figure 7C) that was blocked by curcumin to almost control level, while TT in contrast was less effective.

To this end, we carried out immunoprecipitation (IP) followed by western blot assay using nuclear extracts of senescent cells.

Apigenin and luteolin exert inhibitory effects on 5-cytosine DNMT as shown, using nuclear extracts of KYSE-510 cells [ 122].

Using nuclear extracts of BEAS-2B and NHBE cells, the antibody recognized a single band of about 52 kDa in both western-blot and immunoprecipitation experiments.

Instead of using nuclear extracts, we used proteins translated in vitro from the same mRNA used for the microarray experiments.

EMSA were performed using nuclear extracts as follows: 10 ug of nuclear extract in a 10-ul reaction volume was incubated on ice for 40 min. Double-stranded Oligonucleotide DNA probes, (κB: 5'-AGTTGA GGGGandTTCCCAGGC-3', Oct-1 5'-TGTCGATCGA Oct-1 5'-TGTCGAAA-3') were end-labeled with P-γ, and applied tOct-1 5'-TGTCGAured polyATGCAAATde gel.

Finally, we tested whether these inhibitory effects could be neutralized by an excess amount of SF1 using nuclear extracts prepared from HEK293T cells overexpressing SF1 (Fig. 4E, F).

Time kinetic analyses using nuclear extracts further revealed that the amounts of NF-κB/p65 accumulated in the nucleus over time in response to IL-1β (statistically significant at 30 min; mean increase: 2.36 ± 0.33-fold, n = 3, p < 0.01) but not after TNF-α (Fig.  1c).

In these assays we found increased binding of the oligonucleotide using nuclear extracts from polyphenol-treated cells compared with those from DMSO-treated cells (Fig. 6 A).