We disrupted mtDNA replication in Drosophila using mutations in the two subunits of pol γ, pol γ-β1/β2 (accessory subunit mutant alleles) and tam3/tam9 (catalytic subunit mutant alleles) [9], [27].
We improved the design of all networks using mutations in the coding and operator sequences.
Using mutations in the ATP-binding loop, the kinase activity of STK33 was inferred to be required for the survival of KRAS-dependent cancer cell lines.
Although physical proximity does not necessarily translate into functional significance, in conjunction with the expression patterns and using mutations in the regulatory elements comparatively, the association of DNA sequences can yield insight into molecular mechanisms of transcriptional activation and repression.
Perhaps as a result of this tether interaction with RING1, NMR studies showed poor affinity for parkin with UbcH7 that could be partly enhanced using mutations in the tether to disrupt its association with RING1 [ 34].
Our interest in developing this assay stemmed from prior results with a similar assay system, using mutations in the CYC1 gene (Hampsey 1991), that demonstrated an extremely high-base-pair mutation rate in the absence of MMR, due at least in part to oxidative damage (Earley and Crouse 1998).
Complexes made using mutations in the T-stem of E. coli tRNAVal that hyperstabilize binding to EF-Tu by differing amounts were found to bind E. coli ribosomes and hydrolyze GTP normally, but release from EF-Tu-GDP more slowly than normal and thereby have correspondingly slower rates of dipeptide bond formation.
The sensitivity levels achieved using mutations in this gene were 43% at the highest with a specificity of 93% [ 111– 111].
Here, we use mutations in the dynein heavy chain gene to impair the motor's function, and employ biophysical measurements to demonstrate that cytoplasmic dynein is responsible for the minus end motion of bidirectionally moving lipid droplets in early Drosophila embryos.
We used mutations in the second largest subunit of RNA Pol II 140.
