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According to our probe design and its experimental validation using mutants from the systematic deletion collection, we propose here a complete yeast array in which each of the 5660 probes is specific for a unique target gene.
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Investigations using mutants from species other than S. chacoense should also lead to a better understanding of this MAPKKK family and help revealing their roles.
To determine the polymerase error frequency (Pol EF) within each microsatellite target region, a mutational spectrum of 20−30 mutants was generated for each template, using mutants isolated from two independent polymerase reactions per template.
Considerable studies have been conducted using mutant cells from WHIM patients or a variety of cell lines transfected with truncational mutants of CXCR4 to investigate WHIM pathogenesis.
The functional significance of the 12 variant sequences has been investigated in vitro using mutant proteins expressed from ZIC3 full-length cDNAs containing each relevant mutation (Ware et al., 2004; Chhin et al., 2007).
Next we performed immunoprecipitation using mutant extracts prepared from cultures incubated under restrictive conditions (4 h, at 36°C).
Previous studies have focused on examining the functional effect of single genes on social interactions in D. discoideum, using mutant lines obtained from screening in laboratory strains [ 20, 34, 35].
To elucidate this phenomenon, studies were conducted using deletion mutants from the AOX2 promoter region.
Since there are no vaccines available for the prevention of S. aureus infections, the mutant ATH35L has been investigated as a vaccine target (Menzies and Kernodle 1996; Ragle and Bubeck 2009) and, a previous inflammation study has used mutant ATH35L isolated from crude E. coli (Craven et al. 2009; O'Reilly et al. 1986; Saito et al. 2009).
We used first-order mutants from the RBAC fault domain (Masood et al. 2009) to simulate simple faults and evaluate the effectiveness of each prioritization criteria.
Mitochondrial free radical production and oxygen consumption in dj-1β mutant flies We measured mitochondrial free radical production in 10 day old dj-1β mutant flies using flies from the DAH background as controls.
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