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Newer PCR's using multiple targets and other improved technologies may have better sensitivities.
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This is indeed consistent with our hypothesis that using multiple target fields can help improve the forecasting results.
The poor overlap between different miRNA target prediction algorithms is well known and the lack of rationale behind filtering of targets by using multiple target prediction algorithms has been demonstrated [25].
Although the authors chose to solely investigate Bcl2 as a target of these three miRNAs in mediating the beneficial effects of CR, eight additional shared mRNA targets were found using multiple target-prediction algorithms.
The finding that many miRNAs can target the same KLK and a single miRNA can target more than one kallikrein suggest that miRNAs can exert a 'quantitative' control of kallikreins by using multiple targeting sites in the kallikrein mRNA.
29– 47 The analysis of lymph MM RT-PCR gave more consistent data, which may be related to high volume of lymph fluid in patients with confirmed lymphatic dissemination of the disease (in the blood tumor cells may be present at extremely low concentrations and tumor shedding may be intermittent) and using multiple target markers, including tyrosinase, MART1, and uMAGE.
To predict target genes, which may be co-regulated by the 3 concordant miRs, we used miRGator, an on-line interface that uses multiple target prediction programs.
The miRGator analysis tool (available at http://genome.ewha.ac.kr/miRGator/miRNAexpression.html), an online interface that uses multiple target prediction algorithms, was then used to identify unknown downstream targets of miR-146a.
In contrast to traditional single target therapy, we used multiple-target cocktail therapy to attack common core network markers, i.e., to inhibit several key proteins simultaneously instead of inhibiting only one single target.
To our knowledge, this is the first time that protein binders to a fluorescent protein other than GFP have been made, now allowing approaches using multiple fluorescent targets at the same time.
Using multiple computational target predicting algorithms together with the luciferase-based reporting platform, the interactions between HF-related microRNAs and the 3′ untranslated regions (3′UTRs) of neurohormone associated genes were examined and compared.
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