Sentence examples for using multiple primer sets from inspiring English sources
Our multiplex amplification regime increased the detection as compared to any single primer set indicating the usefulness of using multiple primer sets in initial amplification of target genes.
The combination of 16S rRNA gene amplification using multiple primer sets and the subsequent sequencing of the PCR products by Roche 454 pyrosequencing should therefore be a powerful method to assess the diversity of the microbiota in the GI tract of common carp.
Using multiple primer sets and controls, we found that neither mature BORIS transcripts nor spliced variants are commonly expressed at detectable levels in malignant breast cells or tissues, although endogenous BORIS transcripts can be induced in MCF-7 cells following 5-aza-2'-deoxycytidine treatment.
Finally, we confirmed that histidine auxotrophs consistently harbor the expected deletion by using multiple primer sets querying the presence of starting and recombined junctions.
RNA was subjected to RT-PCR amplification by using multiple primer sets designed for the detection of orthobunyavirus S segment RNA (11 ).
Substantial efforts to amplify R. parkeri by using multiple primer sets (data not shown) from the tick and the patient's blood were not successful.
The array was evaluated using multiple primer pairs.
It should be noted that the use of multiple primer sets biases the number of sequences belonging to the identified taxa.
Despite the advantages obtained, it is worth noting that the use of multiple primer sets can also increase contamination, which is why protocols aimed at minimizing this contamination must be followed.
We therefore performed the main analyses (see below) separately for each genus Four coding regions (rpoB, rpoC1, rbcL and matK) required the use of multiple primer sets for amplification.
In addition, research on updated DNA sequence for PCR primers design and use of multiple primer sets including multiplex PCR test and primers aiming to parasite's DNA inserted into host genome should be encouraged.
