Sentence examples for using multiple primer combinations from inspiring English sources

No difference in size or exon content for scn4ab transcripts was detected when using multiple primer combinations, restriction enzyme digests and sequencing (supplementary material Fig. S8C and Table S1).

Despite the fact that the longer cDNA is likely the functional cox3 mRNA, a genomic copy corresponding to it could not be amplified from genomic DNA using multiple primer combinations (all of which successfully amplified the corresponding fragments in RT-PCRs; data not shown).

The combination of 16S rRNA gene amplification using multiple primer sets and the subsequent sequencing of the PCR products by Roche 454 pyrosequencing should therefore be a powerful method to assess the diversity of the microbiota in the GI tract of common carp.

The array was evaluated using multiple primer pairs.

Multiple primer combinations were used to attempt to amplify the missing genes from each of the species both within individual genes by short PCRs and between genes by long PCRs however we were not able to amplify additional chromosomes from these three species.

For CNVs where the breakpoints were not clear, multiple primer combinations were tested.

Third, the ProPneumo-1 assay is a multiplex assay, which allows for multiple primer combinations in one reaction, instead of multiple monoplex reactions.

PCR was performed using the primer combinations: sense, 5'-TGTGTGCGGAAAGTATGCTGTC-3' and antisense 3'-RACE AAGCAGTGGTATCAACGCAGAGT.

Using four primer combinations we scored 451 polymorphic loci.

No amplification could be obtained with MtCBF13-specific primers from both accessions despite using different primer combinations and PCR conditions.

Initially, our results on twenty-five clinical isolates showed 0 3 polymorphic bands per primer pair while using ten primer combinations.