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This may explain the conflicting data observed and highlights the importance of using multiple cell types when attempting to represent this heterogeneous disease.
The broad applicability of this robotic manipulation technique is demonstrated using multiple cell types on traditional substrates and on open-top microfabricated devices, without requiring modifications to device designs.
In vitro reprogramming of somatic cells to an undifferentiated pluripotent state by viral transfer of defined factors such as SOX2, OCT4, NANOG and LIN28 or SOX2, OCT4, c-Myc, and KLF4[1], [2] has opened the way for the generation of patient-specific human iPSCs using multiple cell types [3], [4].
In comparison, the present study outlined a novel method of in vitro testing using multiple cell types, ENMs, and assays.
Using multiple cell types and combinations, senescent cells did not impact in vivo tumour growth or proliferation.
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Both innate and adaptive immune systems use multiple cell types, a vast array of cell-surface and secreted proteins, and interconnected networks of positive and negative feedback [ 3].
This process can be used with multiple cell types and is capable of reducing the fabrication time while using optimized dispensing parameters for each material.
They have been used with multiple cell types and, as the monolayers heal the wound in a characteristic manner, they have been used to study cell polarization, matrix remodeling, cell migration, and numerous other processes [ 5- 7].
These results indicated that no single cell type would be universally informative for cytotoxicity or apoptosis but that the use of multiple cell types would allow compounds to be binned by their pattern of response (Huang et al. 2008; Xia et al. 2008).
Using photothermally stable SiO2-AuNR, which have enhanced PA signal in comparison to PEGylated AuNRs, and a multispectral PA imaging methodology, our studies demonstrate an approach which can be used to identify multiple cell types within heterogeneous tissue.
Due to tissue-differential and cell type-differential methylation patterns, using a mixture of multiple cell types, as routinely found in human tissues, has long been a critical hurdle in clinical studies [ 22, 23].
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