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The primers and probes were designed using multiple alignments of HPV-6 and HPV-11 partial E6 and E7 sequences that included prototypic and non-prototypic variants.
We also assessed the conservation pattern for these variants using multiple alignments of nine different mammalian mitochondrial sequences.
In this study, we examine ERK1/2 docking with HIV proteins from a drug design perspective using multiple alignments of HIV proteins classified according to subtype.
We located evolutionarily conserved regions by calculating the percent identity in all 2 kb regions spanning the Olig2 promoter using multiple alignments of seventeen species: mouse, rat, rabbit, human, chimp, macaque, dog, cow, armadillo, elephant, tenrec, opossum, chicken, frog, zebrafish, tetraodon and fugu.
Using multiple alignments of complete protein sequences (MACS), we inferred Tex19 and Sectm1 molecular phylogenies.
We employed Sprai [ 26], a new tool for correcting PacBio sequencing errors without other sequencers' reads using multiple alignments of raw PacBio reads.
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The training data set was aligned using multiple alignment of RNAs (MARNA) 29 to obtain the consensus structure with 80% primary and secondary structure identity.
Recently, a new computational method was proposed that identified genome-specifically inserted sequences using multiple alignment of closely related genomes [ 12].
Variant prioritization tools, such as SIFT Ng and Henikoff, 2006, use multiple alignments of homologous proteins and judge a human variant damaging if it alters a highly conserved amino acid.
To reconstruct the evolutionary scenario for viral ligases, we used multiple alignments of the NAD-dependent and ATP-dependent ligases that included the respective protein sequences from the NCLDV, other viruses, and representative archaea, bacteria, and eukaryotes (see Additional File 1 and Additional File 2, respectively), to build maximum likelihood (ML) phylogenetic trees.
Using multiple alignment and sequence analysis of 1134 T-boxes, we determined the specifier codons and classified them into 18 amino acid specificity groups (Additional file 3).
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