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While this is a relatively simple method that does not include compounding influences such as variation among species and latitudes, it nonetheless provides similar estimates to studies using more complex methods (e.g. [13]), and forms a standard baseline for relative predictions of increasing CCS under different scenarios of future habitat recovery.
In both cases, the results matched well with prior investigations using more complex methods on smaller data sets.
Even by relaxing our criteria for differential expression, we were not able to identify any functionally coherent pathways using more complex methods of analysis.
Transgenic silencing mice can be made with conditional constructs using pronuclear injection method [ 2, 43, 44] or using more complex methods such as ES cell screening or lentiviral delivery.
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We have conducted further tests for the proposed method using more complex light sources shown in Fig. 14.
Owing to the nature of the data sources used, it was not feasible to use more complex statistical methods that are more suitable for analysis of trends.
Moreover, the need to use more complex and costly diagnostic methods, especially when the sputum smear is negative, may imply a delay in the diagnosis of TB, as reported in a previous study [ 6].
Use more complex and technical diagnostic methods.
It is uncommon to see papers that report using computational GBA as an important means of identifying genes with a desired function (ignoring the role of sequence similarity, which is no doubt the most-used GBA method; methods that use more complex network-based approaches are our focus here).
Future studies in primary care should preferably combine quantitative and qualitative research methods and use more complex designs to get a better insight into these complex constructs and possibly mediating or moderating factors.
Another method might use more complex regimens, such as sequential therapies, to achieve successful eradication by lower bacterial load.
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