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One milliliter WS1 was diluted tenfold using mobile phase to prepare working standard 2 (WS2, 10 µg/mL).
Concentration of 1.0 mg/mL of MST was dissolved in DMSO then 1 mL of this solution was diluted ten fold using mobile phase to prepare working standard 1 (WS100100 µg/mL).
Concentration of 0.1 mg/mL of IS was prepared in DMSO then 200 µL of this solution was diluted 50-fold using mobile phase to prepare working IS (WI, 2 µg/mL).
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The same procedure was applied to prepare blank using mobile phase instead to confirm the absence of any interference at the retention time of VNT and IS.
Resultant mixtures were subjected to HPAEC-PAD using mobile phase of 100 mM NaOH with 10 mM sodium acetate.
Standard solutions of each was transferred in vials to reach concentration in range of 0.2 1 ppm using mobile phase.
Standard solutions of OpP was transferred in vials to reach final concentrations in range of 100 1000 ppm using mobile phase.
XBridge HILIC column with isocratic elution using mobile phase 10 mM K2HPO4 pH 7.2 acetonitrile (26:74; v/v) was employed.
Ruggedness of the method was determined by using mobile phase components from two different manufactures.
by using mobile phase of 5% acetonitrile (ACN) in 0.1% formic acid (FA).
The %RCP using mobile phase 1 was detected at 99.0 ± 0.9% (n = 23).
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