Sentence examples for using minimal medium from inspiring English sources
Cellulase production was carried out under solid-state fermentation at 45 °C using minimal medium as an initial moistening agent.
By using minimal medium, yeast cells inside the alginate beads were maintained, and its growth was kept at its minimal.
Although the antimicrobial peptides were produced in NB for large scale preparation, the influence of different carbon and nitrogen sources on peptides production was tested using minimal medium.
The yeast cells inside the beads were maintained to be uniform by using minimal medium where growth was at its minimal.
This reporter strain was selected and maintained using minimal medium plates lacking histidine.
The reporter strain was maintained using minimal medium plates lacking uracil.
Whereas we only used minimal medium, yields may be further increased by growth medium optimization and the addition of proper feed supplements (Pal et al. 2006) or altered process conditions and advanced feed strategies that already proved successful for GAP promoter-based protein production processes in P. pastoris (Maurer et al. 2006; Baumann et al. 2008).
After a round of colony purification all bacterial colonies indicated to possess cellulase activity were re-tested for cellulose degrading capacity using minimal growth medium supplemented with CMC.
All cells were routinely cultured in 75 cm2 vented tissue culture flasks (Nunc, UK) using minimal essential medium with Earle's salts supplemented with 1% non-essential amino acids, 2 mM L-glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin and 10% foetal bovine serum.
The cell lines were kept under standard tissue culture conditions using Minimal Essential Medium containing 10% foetal calf serum (FCS).
All cells were routinely cultured in 75 cm vented tissue culture flasks (Nunc, UK) using minimal essential medium with Earle's salts supplemented with 1% non-essential amino acids, 2 mM L-glutamine, 100 U/ml penicillin, 100μg/ml streptomycin and 10% foetal bovine serum.
