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Control groups were injected into both flanks at day 28 using mice that had not been pre-challenged with SCC FAK −/− cells.
They repeated the experiment using mice that had been genetically engineered without one type of receptor.
To drive dysregulated Wnt signalling, we used mice that carry a dominant allele of the β-catenin gene in which exon 3 is flanked by loxP sequences (Harada et al., 1999).
In order to drive deregulated Wnt signaling, we used mice that carry a dominant allele of the β-catenin gene in which exon 3 is flanked by loxP sequences (Harada et al., 1999).
Using mice with lox-p flanked conditional alleles and Tie2-Cre transgenes, it was demonstrated that Gpr124 function is specifically required in the ECs [ 102, 104].
Experiment 2 was performed using mice with loxP sites flanking exon-2 of the Vglut2 gene (n = 7).
Briefly, DNA isolated from conjunctival swabs was amplified using primers that flank the ompA gene.
This was confirmed by PCR amplification and sequencing using primers that flank the IR/LSC boundaries.
The resulting Guca1aCOD3 mice were genotyped using primers that flanked a residual intronic loxP sequence to identify gene-targeted allele; the PCR products from homozygous and heterozygous mice (shown in Figure 1b) were sequenced to determine whether the loxP sequence segregated with the A-to-G transversion.
These F1 mice were genotyped using PCR primers that flanked the targeted exon and the residual loxP sequence; the targeted allele, which included the single surviving intronic loxP sequence, gave a larger amplification product than the wild-type allele (Figure 1).
To test if Jac-A can inhibit K562 tumour cell growth in vivo, we used nude mice that were injected s.c. with K562 cells into the right flank.
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