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Extra- and intracellular lactate levels were quantified using methods reported by us.
Nucleus pulposus cells were isolated by using methods reported by Hiyama et al., 2007 [ 1].
If explicit survival data were not provided, they were calculated from the available numerical data using methods reported by Parmer et al. [ 13].
When these statistical variables were not directly provided in the original articles, they were calculated from available numerical data using methods reported by Parmar et al. [ 16].
Primary ovarian cancer cells were obtained from the ascites of patients undergoing surgery for ovarian cancer and cultured in RPMI 1640 with 10% fetal bovine serum (Invitrogen, CA USA), using methods reported by Szlosarek [ 12].
Nucleus pulposus cells and annulus fibrosus cells were isolated from the lumbar discs of 11-week-old Sprague Dawley rats (n = 32) using methods reported by Hiyama et al. [ 3].
Similar(53)
The constructs were introduced into G. lozoyensis by Agrobacterium-mediated transformation using method reported by Zhang et al. [ 21] with slight modification.
To verify function of the predicted pneumocandin gene cluster, glnrps4 and glpks4 deletion were conducted using method reported by Zhang et al. [ 21].
The aliquots were shipped frozen to the laboratory; p,p′ -DDE, o,p′ -DDT, and p,p′ -DDT were assayed using methods reported previously by Gammon et al. (2002).
Herein, thermally reduced graphite oxide (TRGO) was obtained from thermal reduction of graphite oxide prepared by using the methods reported by Brodie and Hummers.
To investigate the Raman enhancement effect of R6G on these GNP arrays quantitatively, the enhancement factor (EF) values were calculated using the methods reported by Van Duyne [44].
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