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Faecal glucocorticoid metabolite levels were measured using methods involving the use of a corticosterone I125 radioimmunoassay (RIA) kit (MP Biomedicals, Costa Mesa, California, USA) [46], [47].
This choice could be refined using methods involving groups of users, as proposed in previous studies [ 28, 45].
A disadvantage of array CGH is that it cannot detect LOH, which has traditionally been identified using methods involving microsatellites and restriction fragment length polymorphisms (RFLPs) that are not suitable for large-scale analyses (see [42]).
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Orlando picked his stocks by throwing a toy mouse at a grid of companies, while his human opponents possibly used methods involving "research" and "thinking".
However, to design a SERS-based biosensor, almost all currently used methods involve the time-consuming and complicated modification of the metallic nanoparticles with the Raman active dye and biorecognition element, which restricts their widespread applications.
Most commonly used methods involve the addition of exogenous site-specific proteases to cleave the affinity tag off the target protein at engineered sites [2].
The nanoparticles were prepared by modifying a routinely used method involving borohydride reduction of silver nitrate in the presence of stabilizing agents.
One frequently used method involves generating a ROC curve using simple linear regression [ 6, 7].
Another previously used method involved a triad of markers directly placed on the posterior aspect of the pelvis.
Direct standardisation, another frequently used method, involves applying local age-sex specific rates to the age-sex population estimate of a reference, or standard [ 5- 7].
A commonly used method involves amplifying the cp genome by rolling circle amplification and then cloning this product into sequencing vectors.
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