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Those ions are further accelerated in electric field and analyzed using mass detector [15, 67].
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The Phenomenex EZ:faast™ amino acid analysis kit is available for gas (GC) or liquid (LC) chromatographic analysis of amino acids (AA) using mass spectrometry (MS) and other GC detectors.
The GC-MS was carried out using a Perkin Elmer mass detector (model TurboMass) operated at an ionization voltage of 70 eV with a scan range of 20 500 Da.
The analyses were performed on an Agilent 7890A GC system fitted with an HP-5MS-fused silica capillary column (30 m, 0.25 mm i.d., 0.25 μm film; J&W Scientific), connected to an Agilent 5975C inert mass detector using the following method: 5 min at 50 °C, then increasing with 10 °C min 1 to 320 °C, operated in splitless mode (60-s valve time).
Mass detector used in split mode, and helium gas with flow rate of 1.0 ml/min was used as a carrier.
The amino acids were detected using a Micromass Quattro Micro tandem mass detector in MRM mode using positive electrospray ionization, with the following transitions: ornithine, 133.1 → 70.2, arginine 175.2 → 70.2 and citrulline 176.2 → 70.2.
The mass detector used in this analysis was Turbo-Mass Gold-Perkin Elmer and the software adopted to handle mass spectra and chromatograms was a Turbo-Mass ver 5.2.
In some experiments, metabolites were identified by on-line mass spectrometry using a single-stage quadrupole mass detector (Agilent MSD model D) with negative mode atmospheric pressure chemical ionisation, using N2 as the nebulising and drying gas at a flow rate of 5 l min−1 and a nebulising pressure of 55 psi.
Gas chromatograph analysis was periodically performed using PerkinElmer Clarus 500 coupled with mass detector.
The kinetics of GD degradation was studied using a gas chromatography with mass detector (GC MS) and solid-phase microextraction.
Chromatograms were screened for any interferences peaks at retention times of MST or IS, MRM mode in the mass detector was used to raise the specificity of the current method.
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