Sentence examples for using mariner from inspiring English sources

Two in vitro transposition libraries were constructed using mariner transposase, essentially as described by Gaskin and van Vliet [ 79] and Tn7 transposase from New England Biolabs with C. jejuni NCTC11168 genomic DNA.

Prior Tn-seq experiments using Mariner libraries have taken advantage of an engineered MmeI restriction site that cuts 18 20 base pairs away from the recognition site into the genomic DNA [ 27, 30].

"We've got some margin in here and we're using it," Mariner Manager Lou Piniella said.

The eluted peak was collected and analyzed by mass spectrometry using a Mariner System 5220 (Applied Biosystem) spectrometer.

B. fragilis was mutagenized using the mariner transposon vector as described previously [ 25].

Using a mariner transposon approach, A. marginale was transformed and genes encoding TurboGFP and spectinomycin/streptomycin resistance were inserted [ 9].

We have described a method for performing Tn-seq with P. gingivalis using a Mariner mini-transposon and Illumina platform next-generation sequencing.

This group of genes was supplemented with developmental mutants identified using the mariner-based transposable element magellan-4 [14], which was used previously to identify motility genes in M. xanthus [15] [17].

Recently, Yang et al. constructed a co-transposition system in yeast using rice Mariner-like and Stowaway-like elements to demonstrate that the Stowaway-like MITEs could actively transpose [ 16].

Indeed, in D. virilis, no mariner was detected in a BLASTN search using the 36 mariner sequences with default parameters.

In Plasmodium, transposition has been reported using the Drosophila mariner transposable element but the transposition events occurred at a very low frequency independent of transposase [ 17].