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Collecting bright and stable emission from fluorescent organic dyes or nanoparticles using low laser excitation is essential when these fluorophores are used for biological imaging.
So far, only graphitization has been demonstrated using low laser energies (≤1 J/cm2).
The laser hot-wire (LHW) process has the potential to deposit material at high rates using low laser power.
The more active probes can also preclude thermal damage to the samples by using low laser power and permit high-resolution chemical imaging instead of only point spectroscopic measurements.
Bleaching and cell damage were minimized by using low laser levels (1 2%).
A series of images was then collected over time using low laser illumination in order to monitor recovery of the fluorescent protein into the bleached region (Figure 4).
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Since in this measurement we have used low laser power, the map shows the spectra development only in the range from 8.5 to 190 K.
Therefore, it is capable of acceptable SNR using low enough laser intensity to meet the laser safety requirement for maximumpermissible exposure (MPE) in biological media [ 40].
This process is usually carried out using low intensity laser beams and ordinary components such as mirrors and optical fibers.
Further work is being conducted on pore dynamics to properly quantify the resealing time for pores created using low average laser powers.
Though, we can not argue that the therapists were providing inadequate care by not using low level laser, TENS or acupuncture.
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